Study of antimalarial activity by the Homeopathic Complex Canova®, in model murine - Plasmodium berghei

Malaria is caused by four species of Plasmodium which P. falciparum is the most dangerous, is still the most devastating human parasite infection in the world. Drugs, insecticides and vaccines practical, cheap, effective and safe are still needed to fight malaria. In the 1950s, attempts to eradicate this protozoan most of the world have failed, mainly due to development of resistance to insecticides and antimalarial drugs. The search for new therapeutic alternatives is of paramount importance for the development and combating malaria. Thus, this research it is an experimental study using Plasmodium berghei strains for appraisal of any antimalarial effect of a homeopathic immunomodulatory compound, Canova, whose main objective will be to analyze a possible reduction of the parasitemia level in mice infected intentionally. After 4 days of treatment with 0.3 ml dose of Canova® intraperitoneal route the parasitemia was determined and the level of parasite reduction of the mice of the test group was evaluated, obtaining as a result a reduction of 57.5% of the parasite load after 5 days of treatment. According to the results obtained, it is concluded that the immunomodulator Canova has activity against the Plasmodium berghei with a reduction of 57.5% of the parasitological level. However, further studies are needed to assess its clinical efficacy against malaria in humans.

Year of publication: 2016

in: JUAZEIRO DO NORTE - FJN

“In vitro action of the homoeopathic medicine Canova in bone marrow cells of mice"

This study verifies the effects of the homoeopathic composition Canova on cells of the stromal of the bone marrow of mice (supporters) cultivated in vitro. The cells were activated after the treatment and nests of cells appeared (multiplication and differentiation) on the adherent layer.

Year of publication: 2005

in: UFPR

"Action of the homoeopathic medicine Canova in the cicatrization on the back of mice after incision and suture: a macro and microscopic evaluation"

The treatment with Canova accelerated the cicatrization and reduces the inflammation of the incision in the back of mice. It also reduced the cells proliferation, hindering the formation of hypertrophic scar.

Year of publication: 2005

in: UFPR

"Patients HIV/AIDS treated with the homoeopathic medicine Canova, they improve laboratory indexes, clinical and of life" quality”

This study evaluated the longitudinal evolution of patient HIV/AIDS+, after one month and after six months of use of the medicine Canova, concomitant or not with the use of conventional medicines. It demonstrated that the treatment allows an improvement in the following parameters: increase of the appetite and of the available energy for daily routine, decrease of the pain and depression, opportunistic infections decrease, increase of the n° of lymphocytes CD4 and of the total n° of erythrocytes, thus improving the quality of life.

Year of publication: 2005

in: UFPR

“Patients recovery from HIV/AIDS in Botswana, Africa after using homoeopathic medicine Canova"

This study demonstrated that the treatment with the homoeopathic medicine Canova was highly effective in the reduction of the symptomatology and in the improvement of the quality of the patients' life with HIV/AIDS, once it improved parameters as: general perception of pain, appetite, capacity for the accomplishment of small efforts and absenteeism. The results demonstrated significant change in each parameter of life quality evaluated; already in the first month of treatment, and also that these improvements remain after the eighteen months of the use of the medicine Canova.

Year of publication: 2005

in: UFPR

"Evaluation of homoeopathic medicines in peritoneal macrophages of mice"

Study on the effects of the treatment in vitro of seven homoeopathic medicines, widely used around the world, in different potencies (dilutions) on the macrophages. The study concludes that several homoeopathic medicines work through macrophages. Possible route of action of those medicines in the organism would be through Th1 and/or Th2, for instance Belladonna CH 200 stimulates the interferon production (Th1) and Mercurius CH 200 stimulates the production of interleukin 4 (Th2). The homoeopathic medicine Canova has a different action from the medicines of its composition when analyzed isolated or separately. Different potencies from a same substance are different medicines, inducing different answers.

Year of publication: 2005

in: UFPR

"Effects of the Homeopathic Medicine Canova at the Endosomal / Lysosomal system and macrophage activation by Canova medication"

This research demonstrated that macrophages treated have larger capacity of spreading shore and to phagocyte microorganisms. The activity of the phosphatase acid of macrophages treated with the medicine Canova is increased, as well as the endosomal/lysosomal system of these cells. On the other hand, the activity of the enzyme resistant acid phosphatase to the tartrate, decreased with the treatment. These results demonstrate that Canova medicine increases the effectiveness of the treated macrophages.

Year of publication: 2004

in: UFPR

“Chromosomal study of human lymphocytes treated with Canova Medicine and its action in the cellular cycle (G1, S, G2, M)"

This work evaluated human lymphocytes treated with Canova medicine, stimulated by fitohemaglutinina. The study proved the non-toxicity of the medicine. The used methodology evaluated the effect of the medicine Canova on DNA, demonstrating that the medicine neither presents mutagenicity nor genotoxicity.

Year of publication: 2003

in: UFPA

"Imunommodulator effect of Canova Medicine in the Experimental Infection by Leishmania Amazonensis"

This research investigated the effects of Canova medicine in the experimental infection by Leishmania amazonensis in mice. The results in vitro showed that Canova medicine reduced the infection index and stimulated Nitric Oxide’s production. In the infections in vivo in it demonstrated that the treatment with Canova medicine reduced the lesion strongly in the paws of the mice. These results indicate that Canova medicine modulates the experimental infection for Leishmania, controlling the progression of the infection and limiting its spread.

Year of publication: 2003

in: UEM

"Effect of the Canova Medicine on the Leukocyte Parameters in normal mice and bearers of Sarcoma 180"

This study showed that the treatment with Canova on mice sarcoma bearers increased in a significant way the monocytes number and lymphocytes in the outlying blood, mainly B cells, CD4, CD8 and particularly the NK cells.

Year of publication: 2002

in: UFPR

"Histopatologycal study of the Sarcoma 180 on mice treated with homeopathic medicine Method Canova"

This study showed that the medicine reduced the tumor volume on sarcoma bearers animals treated with Canova medicine. The treated tumors developed, in a significant way, a peritumoral leucocitary infiltrated and peritumoral fibrosis. Some remaining nodules, after Histopathological evaluation, demonstrated tumor regression, with absence of cancerous cells and only the presence of a necrotic central area delimited by fibrosis.

Year of publication: 2002

in: UFPR

“Activated Macrophages by Canova Medicine in interaction with Toxoplasma gondii”

This study showed that the homoeopathic medicine Canova acts in the interaction parasite-macrophages, demonstrating that a decrease of the macrophage infection happened for T.gondii when treated in vitro and/or in vivo in with the medicine Canova.

Year of publication: 2002

in: UERJ

"Effects of the Canova medicine on functional aspects of macrophages"

The research demonstrated that macrophages treated with the medicine Canova increased in a significant way the phagocytosis of Trypanosoma cruzi epimastigota and also of Saccharomyces cerevisiae. There was also a small increase Nitric Oxide’s production, which was constant and significant in the presence or not of intracellular parasites.

Year of publication: 2002

in: UFPR

“Structural alterations in Peritoneal Macrophages of Mice Treated with Canova Medicine”

The author demonstrated that, under the action Canova medicine, an alteration in the distribution of some membrane receivers happened and that it reduced the production of the TNF alpha.

Year of publication: 200

in: UFPR